Closed-Loop Stimulation

Sleep in adult C. elegans occurs spontaneously, making timing of individual sleep/wake state transitions unpredictable. We developed a closed-loop system to automatically detect sleep state transitions and subsequently deliver stimuli and record evoked neural responses. This allows users to assess functional consequences of behavioral states without bias in order to identify state-dependent neuromodulation. We published this work here:

The closed-loop system is flexible beyond sleep detection, and can be configured to detect any visible events (such as specific behavior patterns, locations, or optical reporters) and measure corresponding evoked neural responses.

For files, see the Closed-Loop Stimulation repository.

For more information, follow the links below.